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	Provedor de dados BABT (1) Autor Azevedo,Flávia Beatriz Beserra (1) Carloni,Giuliano Guilherme (1) Carvalheira,Lucia Verçosa (1) Palavra-chave Artificial substrata (1) Benthic organisms (1) Colonization (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2006 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 34 Primeira ... 1 2 ... Última Genetic susceptibility to HPV infection and cervical cancer BJMBR Maciag,P.C.; Villa,L.L.. Squamous cell carcinoma of the cervix (SCCC) is one of the leading causes of death in developing countries. Infection with high-risk human papillomavirus (HPV) is the major risk factor to develop malignant lesions in the cervix. Polymorphisms of the MHC and p53 genes seem to influence the outcome of HPV infection and progression to SCCC, although controversial data have been reported. MHC are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in immune regulation, while p53 is a tumor suppressor gene that regulates cell proliferation. The HPV E6 protein from high-risk types binds p53 and mediates its degradation by the ubiquitin pathway. The role of these polymorphisms in genetic susceptibility to HPV... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Cervical cancer; HLA; P53; Polymorphism. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700017 c-Myc protein expression is not an independent prognostic predictor in cervical squamous cell carcinoma BJMBR Brenna,S.M.F.; Zeferino,L.C.; Pinto,G.A.; Souza,R.A.; Andrade,L.A.L.; Vassalo,J.; Martinez,E.Z.; Syrjänen,K.J.. The c-myc protein is known to regulate the cell cycle, and its down-regulation can lead to cell death by apoptosis. The role of c-myc protein as an independent prognostic determinant in cervical cancer is controversial. In the present study, a cohort of 220 Brazilian women (mean age 53.4 years) with FIGO stage I, II and III (21, 28 and 51%, respectively) cervical squamous cell carcinomas was analyzed for c-myc protein expression using immunohistochemistry. The disease-free survival and relapse-rate were analyzed using univariate (Kaplan-Meier) survival analysis for 116 women who completed the standard FIGO treatment and were followed up for 5 years. Positive c-myc staining was detected in 40% of carcinomas, 29% being grade 1, 9% grade 2, and 2% grade 3.... Tipo: Info:eu-repo/semantics/article Palavras-chave: C-myc; Oncogene; Cervical cancer; Prognosis. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400003 Estrogen and progesterone receptors in human papilloma virus-related cervical neoplasia BJMBR Coelho,F.R.G.; Prado,J.C.M.; Pereira Sobrinho,J.S.; Hamada,G.; Landman,G.; Pinto,C.A.; Nonogaki,S.; Villa,L.L.. Estrogen (ER) and progesterone (PR) receptors in the normal uterine cervix, cervical intraepithelial neoplasia and invasive carcinoma were studied in consecutive samples from Hospital do Câncer, São Paulo, between 1996 and 1997. Tissue was collected by removing a fragment of the tumoral area using a 5-mm diameter biopsy punch, followed by removal of a macroscopically normal area as close as possible from the tumor. Histopathological confirmation was obtained for all specimens analyzed. A total of 24 normal tissues, 17 cases of cervical intraepithelial neoplasia and 7 of invasive carcinomas were studied. The ER/PR ratio was determined by immunohistochemistry using monoclonal antibodies specific for each receptor. Adjacent tissue slides were submitted to... Tipo: Info:eu-repo/semantics/other Palavras-chave: Estrogen receptor; Progesterone receptor; Cervical cancer; Cervical intraepithelial neoplasia; Human papillomavirus. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100012 Human papillomavirus infection in Rio de Janeiro, Brazil: a retrospective study BJID Carvalho,M.O.O; Carestiato,F.N; Perdigão,P.H; Xavier,M.P.P.T; Silva,KC; Botelho,M.O; Oliveira,L.H.S.; Cavalcanti,S.M.B.. There is considerable data to support a central role for human papillomavirus (HPV) in the etiology of cervical cancer. More than a 100 HPV types have been described, and 40 have been isolated from benign and malignant genital lesions. Consequently, there is strong motivation to evaluate HPV testing for cervical cancer screening. Few studies concerning the natural history of HPV infection have been conducted in the state of Rio de Janeiro. We determined the prevalence of HPV types in female genital lesions by using Hybrid Capture Assay (HCA) and we retrospectively analyzed the course of HPV infection. Our sample included 788 women attended at Laboratórios Sérgio Franco. The average age of the participants was 29.6 years. HPV prevalence and cytological... Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV; Cervical cancer; Rio de Janeiro; Brazil. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000500007 Epidemiological and functional implications of molecular variants of human papillomavirus BJMBR Sichero,L.; Villa,L.L.. Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; DNA sequencing; Oncogenic potential; Cervical cancer; Immunity; Epidemiology. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600002 The effect of performing corrections on reported uterine cancer mortality data in the city of São Paulo BJMBR Antunes,J.L.F.; Wünsch-Filho,V.. Reports of uterine cancer deaths that do not specify the subsite of the tumor threaten the quality of the epidemiologic appraisal of corpus and cervix uteri cancer mortality. The present study assessed the impact of correcting the estimated corpus and cervix uteri cancer mortality in the city of São Paulo, Brazil. The epidemiologic assessment of death rates comprised the estimation of magnitudes, trends (1980-2003), and area-level distribution based on three strategies: i) using uncorrected death certificate information; ii) correcting estimates of corpus and cervix uteri mortality by fully reallocating unspecified deaths to either one of these categories, and iii) partially correcting specified estimates by maintaining as unspecified a fraction of deaths... Tipo: Info:eu-repo/semantics/article Palavras-chave: Uterine cancer; Cervical cancer; Mortality; Statistical models; Socioeconomic factors; São Paulo. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800012 The environmental cofactors in carcinogenesis in high risk HPV/HIV-positive women BJID Paulo,Michele; Borges,Alex Bittencourt; Duarte,Geraldo; Quintana,Silvana Maria; Montes,Marlise Bonetti Agostinho; Toloi,Maria Regina Torqueti. The objective of the present study was to assess the presence of human papilloma virus (HPV) in HIV-infected women, with comparison between the Papanicolaou cytologic technique and the molecular PCR technique, as well as to determine the type of HPV, to measure cellular immunocompetence and to identify the presence of risk factors for the acquisition of HPV infection. Thirty HIV-infected women were selected. Vaginal and endocervical samples were collected from 27 of them. The smears were examined by 3 experienced cytologists to diagnose the presence of HPV by the Papanicolaou technique and the results were compared to HPV detection and typing by PCR. HPV-infected patients were interviewed in order to identify the presence of risk factors for the... Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV; HIV-infected women; Cervical cancer; Carcinogenesis; Antiretroviral therapy. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200003 Problems and pitfalls in vulvar and cervical cancer sentinel node scintigraphy BABT Sinzinger,Helmut; Granegger,Susanne; Palumbo,Barbara; Palumbo,Renato. After the introduction for penile cancer, the sentinel lymph node imaging is increasingly applied in various types of cancer. After the initial learning phase, 105 patients with vulvar and 24 with cervical cancer have been investigated. In vulvar cancer all the imaged sentinel nodes were discovered by the portable probe intraoperatively. No false negative sentinel node was observed. The most critical issue is the tracer application. Performed strictly intradermally, the sentinel node shows up immediately. Concomitant use of isosulfan blue dye did not improve the results and was stopped therefore. Similarly, more superficial (intra/subendothelial) application brings up better results as compared to deeper injection in cervical cancer patients. No false... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sentinel lymph node; Vulvar cancer; Cervical cancer; Scintigraphy. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700007 p16INK4 expression in precursor lesions of squamous cell cervical cancer related to the presence of HPV-DNA BJMBR Godoy,A.E.G.; Mandelli,J.; Oliveira,F.H.; Calegari,S.; Moura,L.B.; Serafini,E.P.. The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV) infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both... Tipo: Info:eu-repo/semantics/article Palavras-chave: P16INK4; Cervical cancer; Human papillomavirus; Immunohistochemistry. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700006 Human papillomavirus infection in women attended at a cervical cancer screening service in Natal, Brazil BJM Fernandes,Thales Allyrio Araújo de Medeiros; Meissner,Rosely de Vasconcellos; Bezerra,Laelson Freire; Azevedo,Paulo Roberto Medeiros de; Fernandes,José Veríssimo. We analyzed cervical specimens of 202 women, aged 15 to 64 years, attended at Luis Antonio Hospital, Natal, Brazil, to determine the prevalence of HPV and identify the more frequent genotypes and risk factors for HPV infection in women attended at a cervical cancer screening service. Two specimens were collected from each patient: one for cytological examination and the other to detect HPV DNA by PCR, and typing by dot blot hybridization. A total of 54.5% of the sample had normal cytology and 45.5% had cytological alterations. HPV was detected in 24.5% of the cytologically normal women and in 59.8% of those with altered cytology. Both single and double HPV infection increased the likelihood of cytological alterations. Thirteen types of HPV were identified,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Cervical cancer; Epidemiology; Prevalence; Risk factors. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300031 Production of L1 protein from different types of HPV in Pichia pastoris using an integrative vector BJMBR Coimbra,E.C.; Gomes,F.B.; Campos,J.F.; D’arc,M.; Carvalho,J.C.; Mariz,F.C.; Jesus,A.L.S.; Stocco,R.C.; Beçak,W.; Freitas,A.C.. Human papillomavirus (HPV) infection is the most common sexually transmitted disease in the world and is related to the etiology of cervical cancer. The most common high-risk HPV types are 16 and 18; however, the second most prevalent type in the Midwestern region of Brazil is HPV-33. New vaccine strategies against HPV have shown that virus-like particles (VLP) of the major capsid protein (L1) induce efficient production of antibodies, which confer protection against the same viral type. The methylotrophic yeast Pichia pastoris is an efficient and inexpensive expression system for the production of high levels of heterologous proteins stably using a wild-type gene in combination with an integrative vector. It was recently demonstrated that P. pastoris can... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Cervical cancer; Pichia pastoris; L1. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200003 Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA BJM Santos,Carla R.; Franciscatto,Laura G.; Barcellos,Regina B.; Almeida,Sabrina E. M; Rossetti,Maria Lucia R.. This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples. Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV; Cervical cancer; FTA elute; Molecular detection. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100047 Correlation between ebv co-infection and HPV16 genome integrity in Tunisian cervical cancer patients BJM Kahla,Saloua; Oueslati,Sarra; Achour,Mongia; Kochbati,Lotfi; Chanoufi,Mohamed Badis; Maalej,Mongi; Oueslati,Ridha. Infection with high risk Human papillomavirus (HR-HPV) is necessary but not sufficient to cause cervical carcinoma. This study explored whether multiple HR-HPV or coinfection with Epstein-Barr virus (EBV) influence the integration status of HPV16 genome. The presence and typing of HPV in a series of 125 cervical specimens were assessed by polymerase chain reaction (PCR) using the specific primers for the HPV L1 region. As for EBV infection, the viral EBNA1 gene was used for its detection through PCR amplification. Disruption of the HPV E2 gene was assessed by amplification of the entire E2 gene with single set of primers, while E2 transcripts were evaluated by a reverse transcription PCR method (RT-PCR). The overall prevalence of HPVDNA was of 81.8% in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cervical cancer; Epstein-Barr virus; E2 gene; E2 mRNA; High risk human papillomaviruses. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200039 Association between cervical lesion grade and micronucleus frequency in the Papanicolaou test Genet. Mol. Biol. Bueno,Caroline Tanski; Silva,Cláudia Maria Dornelles da; Barcellos,Regina Bones; Silva,Juliana da; Santos,Carla Rossana dos; Menezes,João Evangelista Sampaio; Menezes,Honório Sampaio; Rossetti,Maria Lucia Rosa. The aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p < 0.001). The mean MN frequencies were 0.95 ± 1.12 (mean ± SD) in the control group (n = 223), 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US) (n = 50), 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN) I (n = 52), 5.97 ± 1.83 in CIN II... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cervical cancer; Cervical lesions; Micronucleus; Papanicolaou test. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400004 Expression of TRF2 and its prognostic relevance in advanced stage cervical cancer patients Biol. Res. Ozden,Sevgi; Tiber,Pinar Mega; Ozgen,Zerrin; Ozyurt,Hazan; Serakinci,Nedime; Orun,Oya. BACKGROUND: Telomeres are protective caps consisted of specific tandem repeats (5&#8242;-TTAGGG-3&#8242;). Shortening of telomeres at each cell division is known as "mitotic clock" of the cells, which renders telomeres as important regulators of lifespan. TRF2 is one of the critical members of shelterin complex, which is a protein complex responsible from the preservation of cap structure, and loss or mutation of TRF2 results in DNA damage, senescence or apoptosis. Since cancer is frequently associated with aberrant cell cycle progression, defective DNA repair or apoptosis pathways, TRF2 could be one likely candidate for cancer therapy. Here we investigated the prognostic role of TRF2 levels in cervical cancer patients. Fold-induction rates were... Tipo: Journal article Palavras-chave: Cervical cancer; Radiotherapy; Telomere repeat-binding factors 2; B-cell lymphoma-extra large; Apoptosis. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100055 TT virus in peripheral blood cells from patients with human papillomavirus (HPV): investigating association with cervical carcinoma BABT Suzuki,Patrícia Sayuri; Oliveira,Karen Brajão de; Herrera,Ana Cristina do Amaral; Borelli,Sueli Donizete; Guembarovski,Roberta Losi; Oliveira,Carlos Eduardo Coral de; Oda,Julie Massayo Maeda; Ozawa,Patricia Midori Murobushi; Watanabe,Maria Angelica Ehara. Torque Teno Virus (TTV) presence was investigated in peripheral blood of 117 brazilian women by nested polymerase chain reaction. TTV DNA was observed in 18.6% of healthy donors and in 24.32% Human Papillomavirus (HPV) patients. TTV presence was also investigated in the HPV positive group for comparison between the cervical cancer and noncancerous patients. TTV DNA prevalence was significantly higher among the HPV positive patients with cervical cancer (57.14%) than in HPV noncancerous patients (16.67%). Thus, the presence of TTV infection could be a risk factor for cancer development in the patients presenting HPV-TTV coinfection. Further studies are required to clarify the TTV influence in HPV pathogenesis. Tipo: Info:eu-repo/semantics/article Palavras-chave: TTV DNA; HPV; Cervical cancer. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200010 Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil BJID Entiauspe,Ludmila Gonçalves; Seixas,Fabiana Kömmling; Nunes,Emily Montosa; Rodrigues,Fernanda Martins; Dellagostin,Odir A.; Collares,Tiago; Silveira,Mariângela Freitas da. Background:It is believed that Human Papillomavirus (HPV) and Human Immunodeficiency Virus coinfection contributes to increase the risk for cervical intraepithelial injuries. Several factors may contribute to cervical cancer (CC) development, including genetic variants such as TP53and MDM2 gene polymorphisms.Materials and methods:A hundred HIV-infected women were examined for HPV detection and its genotypes, as well as the frequencies of the SNPs Arg72Pro and SNP309 and their associations with CC risk factors. Nested Polymerase Chain Reaction (nPCR) was used for HPV detection and PCR-RFLP for TP53 and MDM2 SNP309 genotyping.Results:HPV DNA was detected in 68% of samples. A higher frequency of low-risk HPV genotypes (66.7%) was observed when compared to... Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV infection; HIV infection; Cervical cancer; Epidemiology. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600643 Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain BJID Wei,Lanlan; Chu,Ming; Zhang,Qingmeng; Wang,Yan; Shang,Qinglong; Zhang,Yunyan; Zhang,Guangmei. Objectives:To analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12).Methods:The variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4+ and CD8+ in spleen cells was detected with flow cytometry.Results:The titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformation-dependent HPV16... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cervical cancer; Human papillomavirus type 16; Virus-like particle; Cellular immunity; IL-12. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100065 High incidence of oncogenic HPV genotypes found in women from Southern Brazil BJM Entiauspe,Ludmila G; Silveira,Mariângela; Nunes,Emily M; Basgalupp,Suélen P; Stauffert,Dulce; Dellagostini,Odir A.; Collares,Tiago; Seixas,Fabiana K.. Oncogenic HPV genotypes are strongly associated with premalignant and malignant cervical lesion. The purpose was to determine human papillomavirus (HPV) prevalence and genotypes, and to estimate cervical cancer risk factor associations. Cervical samples were obtained from 251 women seeking gynecological care at the Pelotas School of Medicine Clinic. This is a cross-sectional study. HPV-DNA was amplified by nested-PCR using MY09/11 and GP5/6 primers, and the sequencing was used for genotyping. Sociodemographic and behavioral risk factors were obtained by closed questionnaire, and its relationship to HPV infection prevalence were analyzed. Statistical analyses were performed using SPSS 16.0 software, and differences were considered significant at p <... Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV; SDT; Molecular epidemiology; Cervical cancer. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200043 Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines International Journal of Morphology Ili,Carmen G; Brebi,Priscilla; Garcia,Patricia; Leal,Pamela; Lopez,Jaime; Tapia,Oscar; Letelier,Pablo; Weber,Helga; Castillo,Jonathan; Roa,Juan C. Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected... Tipo: Journal article Palavras-chave: Apoptosis; Proliferation; Cervical cancer; SiRNA; C-FLIPL. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036 Registros recuperados: 34 Primeira ... 1 2 ... Última

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